文章摘要
卢燕珊,彭文君,贲永光.超声提取蜂胶黄酮的研究[J].声学技术,2020,39(2):190~194
超声提取蜂胶黄酮的研究
Ultrasonic extraction of flavonoids from propolis
投稿时间:2019-10-01  修订日期:2019-12-27
DOI:10.16300/j.cnki.1000-3630.2020.02.011
中文关键词: 蜂胶黄酮  超声  提取得率  响应面法
英文关键词: propolis flavonoids  ultrasound  extraction yield  response surface methodology
基金项目:农业农村部授粉昆虫生物学重点实验室开放基金(2017MFNZS06)、广东省自然资源厅项目(GDOE2019A27)、广东省科技计划项目(2016A020210133)
作者单位E-mail
卢燕珊 广东药科大学药学院, 广东广州 510006  
彭文君 中国农业科学院蜜蜂研究所农业农村部授粉昆虫生物学重点实验室, 北京 100093 pengwenjun@caas.cn 
贲永光 广东药科大学药学院, 广东广州 510006
中国农业科学院蜜蜂研究所农业农村部授粉昆虫生物学重点实验室, 北京 100093 
biyongguang2002@163.com 
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中文摘要:
      蜂胶黄酮是蜂胶的主要活性成分,具有抗氧化、抗菌等多种功效。优化蜂胶黄酮的超声提取工艺,为蜂胶产品的开发与利用提供了理论依据。首先进行了单因素试验,研究超声功率、超声时间和乙醇浓度这三个因素分别对蜂胶黄酮提取得率的影响。在此基础上,再采用响应面法优化蜂胶黄酮的超声提取条件。研究结果表明,影响蜂胶黄酮提取得率的主次因素依次是超声时间、乙醇浓度和超声功率。当超声功率为150 W、超声时间为16 min、乙醇浓度为86%时,蜂胶黄酮提取得率实际最大可达到21.10%±0.075%(n=3)。经本实验分析可知,湖北孝感蜂胶属于蜂胶中的优等品。相比于其他超声提取蜂胶黄酮的方法,该实验的超声提取方法具有提取温度低、提取时间短的优势。
英文摘要:
      Flavonoids are the main bioactive components in propolis, which exhibit a variety of physiological activities such as anti-oxidative and antibacterial effects. Optimization of ultrasonic extraction of propolis flavonoids will provide a theoretical basis for exploitation and utilization of propolis products. Firstly, single-factor experiments are carried out. The effects of three extraction parameters (ultrasonic power, extraction time and alcohol concentration) on extraction yield of propolis flavonoids are investigated. Secondly, based on the single-factor tests, the ultrasonic extraction conditions of flavonoids from propolis are optimized by using response surface methodology. The experimental results show that the order of factors affecting the extraction yield is extraction time, alcohol concentration and ultrasonic power. The maximum extraction yield of propolis is 21.10%±0.075% (n=3) while ultrasonic power is 150 W, extraction time is 16 min and alcohol concentration is 86%. According to the analysis of this experiment, it is found that the propolis from Xiaogan, Hubei is identified as premium grade product in propolis. Compared with other ultrasonic extraction methods of propolis flavonoids, the ultrasonic extraction method used in this study has the advantages of lower extraction temperature and shorter extraction time.
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