文章摘要
刘冰弥,张闯,李丽,刘洋成,栾佳思,刘宇.超声结合4,4-双甲基姜黄素对DNA损伤的实验研究[J].声学技术,2020,39(5):586~591
超声结合4,4-双甲基姜黄素对DNA损伤的实验研究
Experimental study on DNA damage induced by ultrasound combined with 4, 4-dimethylcurcumin
投稿时间:2019-06-17  修订日期:2019-09-05
DOI:10.16300/j.cnki.1000-3630.2020.05.011
中文关键词: 姜黄素类似物  声敏性质  活性氧  声化学机制  DNA损伤
英文关键词: curcumin analogues  sonosensitizing property  reactive oxygen  acoustochemical mechanism  DNA damaging
基金项目:辽宁省教育厅科学研究经费资助项目(LJC201908)、辽宁省自然基金资助计划(2019-MS-153)、辽宁大学大学生创新创业计划训练项目经费支持。
作者单位E-mail
刘冰弥 辽宁大学药学院, 辽宁沈阳 110036
辽宁新药研发重点实验室, 辽宁沈阳 110036 
 
张闯 辽宁大学药学院, 辽宁沈阳 110036  
李丽 辽宁大学药学院, 辽宁沈阳 110036
辽宁省天然产物制药工程技术研究中心, 辽宁沈阳 110036 
 
刘洋成 辽宁大学药学院, 辽宁沈阳 110036  
栾佳思 辽宁大学药学院, 辽宁沈阳 110036  
刘宇 辽宁大学药学院, 辽宁沈阳 110036
辽宁省天然产物制药工程技术研究中心, 辽宁沈阳 110036 
liuyu0710@163.com 
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中文摘要:
      文章以4',6-二脒基-2-苯基吲哚为荧光探针,通过荧光光谱方法研究了超声结合姜黄素类似物4,4-双甲基姜黄素(dimethylcurcumin,DMCU)对DNA的损伤作用,并采用氧化-萃取分光光度法及活性氧清除剂对超声作用过程中活性氧(Reactive Oxygen Species,ROS)的产生及其种类进行了检测和鉴别。结果表明,与单独DMCU作用或单独超声作用相比,超声与DMCU的协同作用对DNA的损伤效应更强,且损伤程度随超声照射时间和DMCU浓度的增加而增大。此外,DMCU在超声作用下会降解,且DMCU的降解速率和程度与一定条件下的DNA损伤程度有关,说明DMCU声化学反应中活性氧的产生是导致DNA损伤的原因。结果表明DMCU有可能成为一种新结构类型的声敏剂。
英文摘要:
      In order to further confirm the sonosensitizing property of 4,4-dimethylcurcumin (DMCU), by taking the 4',6-diamidino-2-phenylindole (DAPI) as a fluorescent probe, the fluorescence and UV–Vis spectrum method is used to study the damage effect of ultrasonic irradiation combining DMCU on DNA. Meanwhile, the production and types of reactive oxygen species (ROS) during ultrasonic action are detected and identified by oxidation-extraction spectrophotometry and reactive oxygen scavengers. In addition, the effects of ultrasonic irradiation time and DMCU concentrations on the sonodynamic damage of DNA are also considered. The results show that the ultrasound combined with DMCU could damage DNA obviously, and the damage degree of DNA increases with the increase of ultrasonic irradiation time and DMCU concentrations. Additionally, DMCU is degraded under ultrasonic irradiation. The rate and extent of DMCU degradation is related to that of DNA damage under certain conditions, which indicates that the generation of reactive oxygen species in the sonochemical reaction of DMCU is responsible for the damage of DNA. The results of this study can provide a strong evidence for the inference of sonosensitizing effectiveness of DMCU.
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